Pre-Employment Drug Testing
A driver can't perform safety-sensitive functions until you have a verified negative pre-employment drug test (49 CFR 382.301). Test at our clinics, on-site with our mobile collectors across Nebraska and Kansas, or at a partner site nationwide. MRO-verified. $75 clinic / $95 mobile.
DOT pre-employment drug testing is required before a driver first performs safety-sensitive functions (49 CFR 382.301). We test at our Nebraska clinics, on-site, or nationwide — MRO-verified and Clearinghouse-coordinated.
Federal law is unambiguous: no driver may perform safety-sensitive functions until you have a verified negative pre-employment drug test result. Dispatch a driver without it and you've violated 49 CFR §382.301 — and taken on real negligent-hiring exposure.
Three ways to get it done
Test the way that fits the hire: at one of our clinics in Lincoln, Beatrice, or West Omaha; on-site with our own mobile collectors across Nebraska and Kansas when you're onboarding a group; or at a partner collection site anywhere in the country when you're recruiting a driver from out of the area. However it's collected, it runs through the same compliant process.
Compliant, MRO-verified, documented
DOT pre-employment tests are laboratory-based and follow 49 CFR Part 40 — chain of custody, lab analysis, and review by a qualified Medical Review Officer before any result is reported. You get a clear negative verification for the driver's qualification file, typically within 24–72 hours.
Don't forget the Clearinghouse
For CDL drivers, DOT also requires a pre-employment FMCSA Clearinghouse full query before the driver performs safety-sensitive functions. As your C/TPA, we coordinate the query alongside the test, so nothing gets missed at onboarding.
Non-DOT hiring, too
Testing a non-regulated new hire? We run non-DOT panels — from a 4-panel to a 10-panel, instant or lab-confirmed — matched to your policy.
Key Features
Required Before Dispatch (§382.301)
A verified negative is federally required before a driver performs safety-sensitive functions — no conditional start.
Clinic, Mobile & Nationwide Options
Our clinics, on-site mobile collection across NE & KS, or a partner site nationwide for out-of-area recruits.
MRO-Verified Lab Results
Part 40 collection, certified-lab analysis, and Medical Review Officer verification.
Clearinghouse Query Coordinated
We run the required pre-employment FMCSA Clearinghouse full query for CDL hires.
DOT & Non-DOT Panels
DOT 5-panel or non-DOT 4- to 10-panel screens, matched to your policy.
$75 Clinic / $95 Mobile
Transparent per-test pricing; non-DOT instant panels from $55; consortium discounts apply.
Compliance Requirements
Compliance Requirement
The rule — 49 CFR 382.301
An employer must receive a verified negative controlled-substances test result before permitting a CDL driver to perform any safety-sensitive function. There is no grace period and no conditional start on a DOT pre-employment drug test.
The Clearinghouse query — 49 CFR 382.701
Before a CDL driver performs safety-sensitive functions, the employer must also run a pre-employment FMCSA Clearinghouse full query — with the driver's consent — and confirm the driver is not in a prohibited status.
Part 40 process
The test is collected and handled under 49 CFR Part 40 and verified by a Medical Review Officer before the result is reported.
Frequently Asked Questions
A lab-based DOT test is typically verified within 24–72 hours of collection. A non-negative result goes to the Medical Review Officer for review before it is reported.
The DOT 5-panel — marijuana, cocaine, amphetamines, opioids, and PCP — collected under Part 40, analyzed by a certified laboratory, and verified by a Medical Review Officer.
No. DOT requires a verified negative result before a driver performs any safety-sensitive function (49 CFR 382.301) — there is no conditional start.
Yes. A pre-employment FMCSA Clearinghouse full query is required for CDL drivers before they perform safety-sensitive functions. As your C/TPA, we coordinate it alongside the test.
$75 in-clinic or $95 mobile per DOT test; non-DOT instant panels from $55. Consortium members receive discounted rates.
Yes. We schedule the collection at a partner site anywhere in the country — useful when you're recruiting drivers across multiple states.
Yes — 4- to 10-panel instant or lab-confirmed non-DOT screens, matched to your employment policy.
Yes. Consortium members receive reduced pre-employment testing rates along with discounts across all Diversified services.
Related Services
C/TPA & Consortium Program
Diversified Drug Testing is your Consortium/Third-Party Administrator — we run your entire DOT drug and alcohol program. Random pool management, every test type collected by our mobile team across Nebraska and Kansas or in-clinic, plus MRO, SAP, and DER support. $129/year per company.
Post-Accident Testing
When an accident happens, the clock starts: DOT requires alcohol testing within 8 hours and drug testing within 32 (49 CFR 382.303). Our 24/7 dispatch and our own collectors across Nebraska and Kansas get a driver tested — and every attempt documented — so you meet the window and stay defensible.
Reasonable Suspicion Testing
When a trained supervisor observes signs of drug or alcohol use, the test must happen fast. Our collectors dispatch same-day, on-site across Nebraska and Kansas, 24/7 — determination and collection documented to keep it defensible. Supervisors need DOT training first; we offer that too.
Get new drivers cleared before they roll — pre-employment testing at our clinics, on-site, or nationwide, with the Clearinghouse query handled.
Contact our testing specialists to learn how we can support your drug and alcohol testing program.
- C/TPA Program Administration
- 24/7 Post-Accident Response
- 3,000+ Testing Sites Nationwide